The MICZOO2 project focuses on improving our understanding and monitoring of low and mid-trophic levels (LMTL), particularly zooplankton and micronekton, which play a crucial role in ocean ecosystems and the global carbon cycle. These organisms transfer energy from primary producers to higher trophic levels and contribute to carbon sequestration through diel vertical migration. Building on the SEAPODYM-LMTL model, which simulates biomass distribution using ocean currents, temperature, and primary productivity, the project ensures the continuity and enhancement of the MICRORYS global reanalysis (1998–2023). MICZOO2 aims to refine model accuracy by developing ensemble simulations, quantifying uncertainties, and improving physical forcings through targeted case studies. By delivering a state-of-the-art, openly accessible dataset of global biomass densities, the project supports knowledge generation and promotes wider use of ocean data. It contributes to the UN Ocean Decade objectives by strengthening scientific capacity and advancing the sustainable management of marine ecosystems.